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Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
Published on: May 28, 2014
Use of BBL CHROMagar MRSA medium for identification of methicillin-resistant Staphylococcus aureus directly from
John Pape1, Jill Wadlin, Irving Nachamkin
1Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, School of Medicine, 4th Floor, Gates Building, 3400 Spruce St., Philadelphia, PA 19104-4283, USA.
Abstract:
We evaluated the ability of BBL CHROMagar MRSA medium (Becton Dickinson, Sparks, MD) to identify methicillin-resistant Staphylococcus aureus (MRSA) directly upon subculture from positive blood culture bottles. There were 124 MRSA isolates recovered from blood cultures in the study. BBL CHROMagar MRSA medium was highly sensitive (97.6% [121/124] at 18 to 24 h of incubation and 100% [124/124] at 48 h) and 99.9% specific for identifying MRSA from positive blood cultures.
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