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Updated: Aug 7, 2026
A Large Animal Model for Acute Kidney Injury by Temporary Bilateral Renal Artery Occlusion
Published on: February 2, 2021
Protective effects of osajin in ischemia-reperfusion of laboratory rat kidney
L Bartosíková1, J Necas, V Suchý
1Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. bartosikoval@vfu.cz
Abstract:
The aim of this study was to analyze the antioxidative effect of osajin during prophylactic administration. The pathological model for in vivo experiment was the unilateral ischemia-reperfusion of kidney of the laboratory rat. The animals were randomly divided into five groups. Osajin was administrated orally in doses of 5, 10 and 20 mg/kg once a day to three premedicated groups. Placebo--0.5% solution of Avicel--was given to the fourth group and the fifth group was completely intact. The premedication lasted 15 days and subsequently the ischemia of the left kidney was incited in general anaesthesia for 60 min. The reperfusion lasted 10 min and it was finished by blood collection from the left ventricle and the reperfused kidney was recovered. Selected biochemical markers were assessed in blood: superoxide dismutase, glutathion peroxidase, total antioxidative capacity and malondialdehyde. The kidney tissue samples were used for histopathological examination. Laboratory and histopathological results confirmed supposed effects of osajine. The dependence between the effect and the applied dose of osajin was linear. The best biochemical results were reached after administration of osajin at the dose of 5 mg/kg. The best histopathological results were reached after administration of osajin at the dose of 10 mg/kg.
Insights
Osajin demonstrated significant antioxidative effects in a rat kidney ischemia-reperfusion model. Prophylactic administration of osajin protected against oxidative stress, with optimal results observed at specific dosages.
Area of Science:
- Pharmacology
- Nephrology
- Biochemistry
Background:
- Kidney ischemia-reperfusion injury is a significant clinical challenge.
- Oxidative stress plays a critical role in the pathogenesis of this injury.
- Natural compounds are being investigated for renoprotective properties.
Purpose of the Study:
- To evaluate the antioxidative and renoprotective effects of osajin.
- To determine the dose-dependent efficacy of osajin in a prophylactic setting.
- To assess osajin's impact on biochemical markers and kidney histology.
Main Methods:
- A rat model of unilateral kidney ischemia-reperfusion was established.
- Osajin was administered orally at doses of 5, 10, and 20 mg/kg for 15 days.
- Biochemical markers (superoxide dismutase, glutathione peroxidase, total antioxidant capacity, malondialdehyde) and histopathology were assessed.
Main Results:
- Osajin administration significantly improved biochemical markers and reduced kidney damage.
- A linear dose-dependent relationship was observed between osajin dose and its effects.
- The 5 mg/kg dose yielded the best biochemical outcomes, while the 10 mg/kg dose showed the best histopathological results.
Conclusions:
- Osajin exhibits significant prophylactic antioxidative and renoprotective effects.
- The optimal dose of osajin for biochemical and histopathological protection varies.
- Osajin represents a potential therapeutic agent for preventing kidney ischemia-reperfusion injury.
