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Updated: Aug 7, 2026

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
Published on: January 26, 2017
Tandem electric field gradient focusing system for isolation and concentration of target proteins
Shu-Ling Lin1, Yuanyuan Li, H Dennis Tolley
1Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
Abstract:
Two electric field gradient focusing (EFGF) systems, one based on a hollow dialysis fiber and the other based on a shaped ionically conductive polymer were serially integrated to trap and concentrate selected proteins while simultaneously desalting and removing other unwanted proteins from the sample. A series of experiments were performed to test the EFGF systems individually and after integration. Online concentration of amyloglucosidase indicated a concentration limit of detection of approximately 20 ng mL(-1) (200 pM) from a sample volume of 100 microL. Concentration of human alpha1-acid glycoprotein with simultaneous removal of human serum albumin was also demonstrated. Elimination of small buffer components while concentrating trypsin inhibitor, and selective concentration and separation of myoglobin from a mixture were performed using the integrated EFGF system.
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