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Published on: March 10, 2021
[Major constituent proteins in donkey hide and their interaction]
Hao Li1, Mei-juan Huang, Shao-quan Zhang
1Institute of Biotechnology, Fuzhou University, China.
Summary
Donkey hide contains collagen and significant amounts of donkey serum albumin. This finding provides insights into the biochemical basis of Ejiao, a traditional Chinese medicine used for blood-related conditions.
Area of Science:
- Biochemistry
- Traditional Chinese Medicine
- Proteomics
Context:
- Ejiao, a traditional Chinese medicine derived from donkey hide, is widely used for blood-related ailments.
- Understanding the protein composition of donkey hide is crucial for elucidating Ejiao's therapeutic mechanisms.
Purpose:
- To identify and quantify the major protein constituents of donkey hide.
- To investigate the role of these proteins in Ejiao's medicinal efficacy.
Summary:
- This study analyzed donkey hide proteins using TriPure isolation reagent and sodium dodecyl sulfate (SDS) methods.
- Collagen alpha1 (I), collagen alpha2 (I), and notably, donkey serum albumin (up to 20-25%) were identified as major components.
- Further analysis revealed complex products of collagen and serum albumin, with molecular weights around 200 kDa.
Impact:
- Identifies donkey serum albumin as a key protein in donkey hide, offering a biochemical clue to Ejiao's blood-related therapeutic effects.
- Provides a foundation for further research into the specific pharmacological actions of Ejiao components.
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