Immunohistochemistry and quantitative analysis of protein expression

Area of Science:

• Pathology
• Biomarker Analysis
• Molecular Diagnostics

Background:

• Immunohistochemistry (IHC) is vital in pathology, with increasing use for companion diagnostics in targeted therapeutics.
• Current IHC assessment relies on subjective interpretation, hindering the accuracy needed for precise diagnostics.
• Emerging companion diagnostics necessitate more objective methods for in situ protein concentration measurement.

Purpose of the Study:

• To review the current state of immunohistochemistry.
• To discuss available devices for quantitative in situ protein expression assessment.

Main Methods:

• Literature review
• Internet search
• Information from device vendors

Main Results:

• Efforts to quantify IHC have a long history but lack broad acceptance.
• Objective accuracy improvements have not significantly outperformed traditional visual analysis.
• Quantitative platforms, especially for multiplexed analysis, are expected to see increased use.

Conclusions:

• Growing demand for complex companion diagnostics will drive adoption of quantitative IHC platforms.
• Quantitative methods are essential for outcomes unachievable by traditional visual assessment.
• Multiplexed analysis capabilities will be a key driver for quantitative platforms.