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Effect of UV-irradiation on rotavirus
Y A Smirnov1, S P Kapitulets, N N Amitina
1D. I. Ivanovsky Institute of Virology of the U.S.S.R. AMS, Moscow.
Abstract:
The effect of UV-irradiation on SAll rotavirus infectivity was followed. The time course of infectivity inactivation in general showed an one-hit pattern. Two basic effects of UV-irradiation on virus particles were investigated: the phenomenon of RNA-protein linkages and the formation of uracil dimers. To determine the number of uridine dimers, 3H-uridine labelled purified rotavirus was exposed to UV-irradiation, subsequently the RNA was extracted and analysed by ascending paper chromatography. Formation of photodimers was found to be an important mechanism of rotavirus inactivation at conventional UV-irradiation; the RNA-protein linkages were registered at high irradiation doses only.