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Epistasis

Epistasis

In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...

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Related Experiment Video

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Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

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Published on: January 15, 2016

A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit

Kenneth Manning¹, Mahmut Tör, Mervin Poole

¹Warwick Horticulture Research International (HRI), University of Warwick, Wellesbourne, Warwick CV35 9EF, UK.

Nature Genetics

|July 13, 2006

Summary

The colorless non-ripening (Cnr) mutation in tomato is caused by an epigenetic change in an SBP-box gene promoter, revealing its critical role in fruit ripening and cell adhesion.

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Area of Science:

Plant Biology
Genetics
Epigenetics

Background:

The Colorless non-ripening (Cnr) mutation in tomato affects fruit ripening and cell adhesion.
The genetic basis and identity of the Cnr gene were previously unknown.

Purpose of the Study:

To identify the gene responsible for the Cnr mutation.
To understand the molecular mechanism underlying the Cnr phenotype.

Main Methods:

Positional cloning to locate the Cnr gene.
Virus-induced gene silencing to confirm gene function.

Main Results:

An SBP-box (SQUAMOSA promoter binding protein-like) gene was identified at the Cnr locus.
The Cnr phenotype arises from a spontaneous epigenetic alteration in the SBP-box gene's promoter.
This epimutation is a rare event in plants.

Conclusions:

SBP-box genes are crucial for normal tomato fruit ripening.
Epialleles play a significant role in plant development and natural variation.