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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Amplification of DNA encoding entire type I polyketide synthase domains and linkers from streptomyces species
Jo-Anne Chuck1, Catherine Dunn, Fe E C D Facultad
1School of Natural Sciences, University of Western Sydney, Parramatta Campus, Locked Bag 1797, Penrith South, DC, 1797 NSW, Australia. j.chuck@uws.edu.au
Abstract:
Polyketides are a group of bioactive compounds from bacteria, plants, and fungi. To increase the availability of analogs for testing, the active sites of polyketide synthases are often substituted with homologous domains having altered substrate specificities. This study reports the design of polymerase chain reaction primers that enables isolation of entire active site domains from type I polyketide synthases with native interdomain linkers. This bypasses the need for further genetic screening to obtain functional units for use in genetic engineering. This is especially important in bioprospecting projects exploring new environments for bioresources.
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