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Related Experiment Videos

Diagnostic tests based on human basophils: potentials, pitfalls and perspectives.

Jörg Kleine-Tebbe1, Stephan Erdmann, Edward F Knol

  • 1Allergy and Asthma Center Westend, Berlin, Germany. kleine-tebbe@allergie-experten.de

International Archives of Allergy and Immunology
|July 14, 2006
PubMed
Summary

Basophil activation tests are valuable for allergy diagnosis but show high variability. Careful allergen evaluation and experienced laboratory performance are crucial for accurate results, avoiding false negatives.

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Area of Science:

  • Immunology
  • Allergy Diagnostics

Background:

  • Human basophils are key in immediate hypersensitivity, releasing mediators upon allergen exposure.
  • Diagnostic tests measure basophil mediators or surface markers (CD63, CD203c) after stimulation.
  • Significant variability exists in basophil reactivity and sensitivity among donors and antigens.

Purpose of the Study:

  • To analyze the variability in basophil activation tests.
  • To highlight factors influencing basophil sensitivity and reactivity.
  • To provide recommendations for optimizing diagnostic utility.

Main Methods:

  • Review of diagnostic tools measuring basophil activation.
  • Analysis of factors affecting basophil response (IgE receptors, IgE levels).
  • Discussion of diagnostic studies in hymenoptera, food, and drug allergies.

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Main Results:

  • Basophil donor and antigen variability impacts reactivity and sensitivity.
  • Intrinsic sensitivity, influenced by IgE receptor numbers and IgE ratios, affects dose-response curves.
  • Standardized single-concentration testing can lead to false-negative results.

Conclusions:

  • Pre-evaluation of each allergen's stimulation range is necessary for accurate basophil testing.
  • Positive controls (anti-IgE) are recommended, acknowledging non-responder populations.
  • Basophil tests are indicated for specific allergy cases and require expert laboratory execution.