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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Alternative splicing: new insights from global analyses.

Benjamin J Blencowe1

  • 1Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, Centre for Cellular and Biomolecular Research, Donnelly CCBR Building, University of Toronto, Toronto, ON M5S 3E1, Canada. b.blencowe@utoronto.ca

Cell
|July 15, 2006
PubMed
Summary
This summary is machine-generated.

Alternative splicing generates significant proteomic diversity in animals. This review covers current research on identifying transcript variants, their roles in health and disease, and their coordinated regulation.

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Last Updated: Jul 11, 2026

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Published on: December 9, 2016

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Molecular Biology
  • Genomics
  • Proteomics

Background:

  • Alternative splicing is a key mechanism for generating proteomic and functional diversity in metazoan organisms.
  • Sequence and microarray data analyses highlight the significant role of alternative splicing.

Purpose of the Study:

  • To review recent progress in understanding alternative splicing.
  • To establish the repertoire of functionally relevant transcript variants.
  • To explore the roles of these variants in normal and disease physiology.
  • To understand the global coordination of alternative splicing for cell- and tissue-specific functions.

Main Methods:

  • Analysis of sequence data.
  • Analysis of microarray data.
  • Review of recent scientific literature.

Main Results:

  • Alternative splicing significantly contributes to proteomic diversity in metazoans.
  • Ongoing efforts focus on identifying functional transcript variants and their physiological roles.
  • Research is exploring the global coordination of alternative splicing.

Conclusions:

  • Alternative splicing is crucial for biological complexity in metazoans.
  • Further research is needed to fully characterize transcript variants and their regulatory networks.