Comparison of techniques to determine the clearance of ruminal volatile fatty acids

The objective of this experiment was to compare measurements of fractional clearance rates obtained by using an unlabeled valerate-CoEDTA technique with measurements obtained by using a (13)C-labeled volatile fatty acids (VFA) technique. The exponential decay rate of the (13)C/(12)C ratio after pulse-dosing (13)C-acetate, (13)C-propionate, or (13)C-butyrate into the rumen was compared with the decay rate of rumen valerate concentration following a simultaneous pulse dose. The unlabeled valerate, CoEDTA, and each labeled VFA, one at a time, were concurrently mixed with the evacuated ruminal content of 6 lactating cows in two 3 x 3 Latin squares. The clearance of VFA by passage to the omasum was assumed to be equivalent to the decay in ruminal Co concentration and was around 50% of the total clearance. Acetate, propionate, and butyrate had similar fractional clearance rates (31.2, 33.4, 30.4%/h, respectively), but propionate had a higher absorption rate (19.2%/h) than butyrate (14.2%/h). Linear regression determination coefficients using the valerate clearance rate as an estimator for acetate, propionate, and butyrate rumen clearance were 0.51, 0.56, and 0.99, respectively. In a second experiment, the (13)C-valerate fractional clearance rate estimate (33.7%/h) was similar to the estimate obtained with unlabeled valerate (35.0%/h) by the valerate-Co technique. No (13)C enrichment of rumen microbes was noted 4 h after the intraruminal infusion of (13)C-valerate. Fractional VFA absorption rate estimates obtained in both techniques were similar, although both were lower than estimates reported in the literature by other methods.