Related Experiment Video
Updated: Aug 7, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Electrochemical biosensor evaluation of the interaction between DNA and metallo-drugs
Marco Mascini1, Graziana Bagni, Maria Letizia Di Pietro
1Dipartimento di Chimica, Università di Firenze, Polo Scientifico Universitario, Via della Lastruccia 3, 50019, Sesto Fiorentino, FI, Italy.
Abstract:
Electrochemical techniques were used to study the interaction between a panel of antiproliferative metallo-drugs and double-stranded DNA immobilized on screen-printed electrodes as a model of the analogous interaction occurring in solution. The propensity of a given metal drug to interact with DNA was measured as a function of the decrease of guanine oxidation signal, which was detected by square wave voltammetry. Estimates of variations in experimental parameters, such as the concentration of complexes, time following dissolution (ageing time) and the presence of chloride, are provided.
Related Concept Videos
Microbial Biosensors
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...

