Expression, purification, crystallization and preliminary crystallographic analysis of the human intracellular
De-Feng Li1, Yun-Feng Li, Qiu-Hua Huang
1Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, People's Republic of China.
Protein and Peptide Letters
|July 18, 2006
Abstract:
The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapour-diffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 A resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 A, beta = 112.99 degrees and three molecule per asymmetric unit.
