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Updated: Aug 7, 2026

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Published on: May 15, 2019
Dietary fatty acids modulate host bacteriocidal response,microbial translocation and survival following blood
L Gianotti1, J W Alexander, T Eaves-Pyles
1University of Cincinnati College of Medicine, Department of Surgery, Transplantation Division, and Shriners BurnsInstitute, Cincinnati, Ohio, USA.
Abstract:
The effect of dietary lipids on bacterial translocation, killing of translocated organisms and host survival was studied in a burned animal model. Balb/c mice were fed with one of the three experimental AIN-76A diets (containing 15% of energy from fish oil, safflower oil or a 50:50 mixture), AIN-76A without added lipids or a nonpurified stock diet. All animals were transfused on day 10. On day 15, the animals were gavaged with 10(10) 14C radiolabelled Escherichia coli and given a 20% burn injury. Survival was 84% in the fish oil group versus 36% in the safflower oil and 50:50 diet groups, and 25% and 20% in the two control groups (P < 0.0001). The numbers of viable translocating bacteria were reduced in all tested organs in the fish oil groups compared to the other groups. It is concluded that a diet enriched in fish oil has beneficial effects during gut-derived sepsis.
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