Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput approaches to dissecting MAPK signaling pathways.

Adam Friedman1, Norbert Perrimon

  • 1Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

Methods (San Diego, Calif.)
|July 18, 2006
PubMed
Summary

High-throughput RNAi screening enables discovery of new components in mitogen-activated protein kinase (MAPK) pathways. This study details methods for assaying MAPK and receptor tyrosine kinase (RTK)/extracellular signal-regulated kinase (ERK) signaling, aiding future functional characterization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Severe psoriasis relapse following tralokinumab therapy for atopic dermatitis.

JAAD case reports·2026
Same author

An Update on Common Genodermatoses Not to be Missed.

Journal of drugs in dermatology : JDD·2026
Same author

Evaluation of Dermatology Procedure Perceptions and Seeking in LGBTQ+ Patients.

Journal of drugs in dermatology : JDD·2026
Same author

Decoding Sensitive Skin Syndrome: International Expert Advisory Insights on Management From India and the United States of America.

Journal of drugs in dermatology : JDD·2026
Same author

Response to van Rooij et al., "Clinical Context and Comparative Interpretation of Dermatologic Disease Burden in People With HIV in the Modern Antiretroviral Era".

Journal of the American Academy of Dermatology·2026
Same author

JAAD Game Changers: Early postoperative water exposure does not increase complications in cutaneous surgeries: a randomized, investigator-blinded, controlled trial.

Journal of the American Academy of Dermatology·2026

Area of Science:

  • Cellular signaling pathways
  • Functional genomics
  • Molecular biology

Background:

  • Mitogen-activated protein kinase (MAPK) pathways are crucial for cellular processes.
  • Previous studies utilized genetic and biochemical methods to identify core signaling components.
  • High-throughput assays offer unbiased, functional genomic insights into pathway regulation.

Purpose of the Study:

  • To describe high-throughput screening approaches for identifying novel MAPK pathway components.
  • To detail methods for assaying the receptor tyrosine kinase (RTK)/extracellular signal-regulated kinase (ERK) pathway.
  • To provide strategies for managing large datasets for future in vivo studies.

Main Methods:

  • Utilized genome-wide RNA interference (RNAi) libraries for screening in cell culture.

Related Experiment Videos

  • Employed phospho-specific antibody-based readouts to assess pathway activity.
  • Developed secondary validation screens and data management techniques.

    • Main Results:

    • Demonstrated the feasibility of high-throughput screening for MAPK pathway discovery.
    • Established a phospho-specific antibody assay for RTK/ERK pathway activity.
    • Outlined methods for managing and analyzing large-scale functional genomic data.

    Conclusions:

    • High-throughput RNAi screening is a powerful tool for discovering novel components in signaling pathways like MAPK.
    • The described methods facilitate unbiased functional genomic analysis of RTK/ERK signaling.
    • The study provides a framework for future in vivo functional characterization of identified components.