Hemifusion arrest by complexin is relieved by Ca2+-synaptotagmin I
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Updated: Jan 30, 2026

Establishment of Deep Hypothermic Circulatory Arrest in Rats
Published on: December 16, 2022
Johanna R Schaub1, Xiaobing Lu, Blair Doneske
1Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street MS-140, Houston, Texas 77005, USA.
Abstract:
Synaptic transmission relies on an exquisitely orchestrated series of protein-protein interactions. Here we show that fusion driven by neuronal SNAREs is inhibited by the regulatory protein complexin. Furthermore, inner-leaflet mixing is strongly impaired relative to total lipid mixing, indicating that inhibition by complexin arrests fusion at hemifusion. When the calcium sensor synaptotagmin is added in the presence of calcium, inhibition by complexin is relieved and full fusion rapidly proceeds.
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