Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nanog retrotransposed genes with functionally conserved open reading frames.

Morag Robertson1, Frances Stenhouse, Douglas Colby

  • 1Centre Development in Stem Cell Biology, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, EH9 3JQ, Scotland.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|July 18, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Small changes in hand height alter absorbance, but not pulsation, in the finger pulse plethysmograph.

Physiological measurement·2026
Same author

Harnessing the murine inner cell mass mechanical environment enhances derivation of in vitro nascent primitive endoderm precursor cells.

Development (Cambridge, England)·2026
Same author

Correction: Tracking early mammalian organogenesis - prediction and validation of differentiation trajectories at whole organism scale.

Development (Cambridge, England)·2026
Same author

Isolation and characterization of a novel glutamate-gated chloride channel subunit (GLC-2) from the canine heartworm Dirofilaria immitis.

Molecular and biochemical parasitology·2026
Same author

OTX2 controls chromatin accessibility to direct somatic versus germline differentiation.

EMBO reports·2025
Same author

Genenames.org: the HGNC and PGNC resources in 2026.

Nucleic acids research·2025

New Nanog gene copies, NanogPc and NanogPd, were found in mice. These functional retrogenes are highly similar to Nanog, potentially causing misattribution of gene expression in stem cell research.

Area of Science:

  • Genetics and Genomics
  • Developmental Biology
  • Molecular Biology

Background:

  • The Nanog gene is crucial for maintaining pluripotency in embryonic stem cells.
  • Retrogenes are copies of genes that have been reverse transcribed from RNA and inserted elsewhere in the genome.

Purpose of the Study:

  • To identify and characterize novel retrotransposed copies of the Nanog gene in mice.
  • To assess the functional activity and evolutionary origin of these Nanog retrogenes.

Main Methods:

  • Bioinformatic analysis to identify Nanog retrogenes (NanogPc, NanogPd) on mouse chromosomes.
  • Protein-coding sequence comparison with the original Nanog gene.
  • Functional assay in embryonic stem cells to test protein activity.

Related Experiment Videos

Main Results:

  • Two novel Nanog retrogenes, NanogPc and NanogPd, were identified on mouse chromosomes 4 and 7.
  • NanogPc and NanogPd are highly homologous (98%) to Nanog and encode functional proteins.
  • These retrogenes likely arose from a common progenitor and retrotransposition events are ongoing.

Conclusions:

  • The high similarity of NanogPc and NanogPd to Nanog could lead to misinterpretation of gene expression studies.
  • Understanding Nanog retrogenes is important for accurate research on pluripotency and early development.