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Updated: Aug 7, 2026

Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea
Published on: December 23, 2022
[Study on the chemical substrates of SFE extract from Rhizoma Cyperi]
Yi-Fan Feng1, Xiao-Ling Guo, Qing Meng
1Guangdong College of Pharmacy, Guangzhou 510224, China. fengyifan@21cn.net
Objective:
To extract the effective part of Rhizoma Cyperi and study the chemical constituents of diffrent parts.
Methods:
The extract of Rhizoma Cyperi was obtained by SFE and dealed with molecular distillation, then the effective components was enriched. The chemical compositions were separated and identified by GC/MS.
Results:
The content of volatile oil in the extract upgraded from 43.2% to 86.0% by molecular distillation, and the relative amount of cyperene and (+) -alpha-cyperone from 20.2% to 38.6%. Fatty acids was not found in Rhizoma Cyperi-MD1.
Conclusion:
The SFE of Rhizoma Cyperi could be refined with molecular distillation. The active ingredients, such as volatile oil and alpha-cyperolone, were remained effectively. The ineffective ingredients fatty acids were removed.