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Related Experiment Videos

A large-scale, high-efficiency and low-cost platform for structural genomics studies.

Xiao Dong Su1, Yuhe Liang, Lanfen Li

  • 1National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, People's Republic of China. su-xd@pku.edu.cn

Acta Crystallographica. Section D, Biological Crystallography
|July 21, 2006
PubMed
Summary

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A new structural genomics platform at Peking University processes 2000 genes annually for structural and functional analyses. This high-efficiency, low-cost system aims to determine protein structures affordably, advancing drug design and understanding of key bacterial pathogens.

Area of Science:

  • Structural Biology
  • Genomics
  • Biotechnology

Background:

  • Established a large-scale, high-efficiency, low-cost structural genomics platform at Peking University.
  • The platform utilizes Beckman Coulter Biomek FX and custom automation systems.
  • Aims to process up to 2000 genes per year for structural and functional analyses.

Purpose of the Study:

  • To develop and implement a cost-effective platform for structural genomics.
  • To accelerate the determination of protein structures for research and drug design.
  • To target key genes from model organisms like Bacillus subtilis and pathogens such as Streptococcus mutans.

Main Methods:

  • Automated gene selection and processing for structural and functional studies.
  • Utilized macromolecular crystallography beamline at Beijing Synchrotron Radiation Facility.

Related Experiment Videos

  • Employed SAD/MAD phasing techniques for structure determination.

    • Main Results:

    • Determined 40 independent protein structures, including 11 novel structures (<30% sequence identity).
    • Successfully processed over 470 Bacillus subtilis and 1200 Streptococcus mutans proteins.
    • Identified genes relevant to metabolism and potential drug targets.

    Conclusions:

    • The genomic approach is successfully adapted for a moderately funded structural biology program.
    • The platform significantly enhances protein structure production efficiency.
    • The goal is to achieve a protein structure determination cost of approximately $10,000.