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Updated: Aug 7, 2026

05:57
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Microarrays of heparin oligosaccharides obtained by nitrous acid depolymerization of isolated heparin
Jose L de Paz1, Dorothe Spillmann, Peter H Seeberger
1Laboratory for Organic Chemistry, Swiss Federal Institute of Technology (ETH) Zürich, Wolfgang-Pauli-Str. 10, HCI F315, 8093, Zürich, Switzerland.
Abstract:
Heparin oligosaccharides derived by nitrous acid depolymerization of heparin have been immobilized on amine-coated glass slides. The formation of a Schiff base creates heparin chips that are a suitable platform for the high-throughput analysis of carbohydrate-protein interactions.

