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Determination of organochlorine and pyrethroid pesticides in fruit and vegetables using solid phase extraction
Zawiyah Sharif1, Yaakob Bin Che Man, Nazimah Sheikh Abdul Hamid
1National Public Health Laboratory, Ministry of Health, Malaysia Lot 1853, Kpg. Melayu Sungai Buloh, 47000 Sungai Buloh, Selangor, Malaysia.
Abstract:
A method to determine six organochlorine and three pyrethroid pesticides in grape, orange, tomato, carrot and green mustard based on solvent extraction followed by solid phase extraction (SPE) clean-up is described. The pesticides were spiked into the sample prior to analysis, extracted with ethyl acetate, evaporated and reconstituted with a solvent mixture of acetone:n-hexane (3:7). Three different sorbents (Strong Anion Exchanger/Primary Secondary Amine (SAX/PSA), Florisil and C18) were used for the clean-up step. Pesticides were eluted with 5mL of acetone:n-hexane (3:7, v/v) and determined by gas chromatography and electron-capture detection (GC-ECD). SAX/PSA was the sorbent, which provided chromatograms with less interference and the mean recoveries obtained were within 70-120% except for captafol. The captafol recoveries for grape were within acceptable range with C18 clean-up column.
