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Updated: Aug 7, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
Published on: November 12, 2012
A strategy for extracting and analyzing large-scale quantitative epistatic interaction data
Sean R Collins1, Maya Schuldiner, Nevan J Krogan
1Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California-San Francisco and California Institute for Quantitative Biomedical Research, San Francisco, California 94143, USA. src.science@gmail.com
Abstract:
Recently, approaches have been developed for high-throughput identification of synthetic sick/lethal gene pairs. However, these are only a specific example of the broader phenomenon of epistasis, wherein the presence of one mutation modulates the phenotype of another. We present analysis techniques for generating high-confidence quantitative epistasis scores from measurements made using synthetic genetic array and epistatic miniarray profile (E-MAP) technology, as well as several tools for higher-level analysis of the resulting data that are greatly enhanced by the quantitative score and detection of alleviating interactions.
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