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[Sequence analysis of ITS2 and CO1 genes of Paragonimus harinasutai]
Bao-zhen Qian1, H Sugiyama, J Waikagul
1Institute of Bio-engineering, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
Objective:
To identify Paragonimus harinasutai from Ninghai, Zhejiang Province, China.
Methods:
Metacercariae were collected from the crabs Sinopotamon chekiangenes in Xixi village of Ninghai County for ITS2 sequence analysis, CO1 sequence analysis and endonuclease BsaHI and StuI analysis by PCR-RFLP. Results The fingerprintings of PCR-RFLP were virtually same to the isolate from Thailand (Nakorn-nayok). The ITS2 sequence with 366 bp and CO1 sequence with 390 bp of the metacercariae collected from Ninghai revealed a nucleotide identity 95.6% and 89.5% respectively to the Thai isolate.
Conclusion:
The study confirmed that Paragonimus harinasutai is present in Ninghai, China, with certain variation on molecular biology in comparison to the Thai isolate.
