Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA polymorphisms within Spanish Trichinella isolates.

T Garate1, E Albarran, F Bolas-Fernandez

  • 1Division of Immunology, National Institute for Medical Research, London, UK.

Parasitology Research
|January 1, 1991
PubMed
Summary

DNA analysis of 13 Spanish Trichinella isolates revealed two distinct groups. However, restriction-fragment polymorphism did not further differentiate these Trichinella groups, indicating limited genetic variation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunoproteomic analysis of Trichinella spiralis larval crude antigens recognized by sera from patients with trichinellosis after treatment with albendazole.

Tropical biomedicine·2021
Same author

Impact of the GK-1 adjuvant on peritoneal macrophages gene expression and phagocytosis.

Immunology letters·2018
Same author

Intraperitoneal administration of the anti-IL-23 antibody prevents the establishment of intestinal nematodes in mice.

Scientific reports·2018
Same author

Present status of laboratory diagnosis of human taeniosis/cysticercosis in Europe.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2017
Same author

Self-adjuvanting C18 lipid vinil sulfone-PP2A vaccine: study of the induced immunomodulation against <i>Trichuris muris</i> infection.

Open biology·2017
Same author

Human dirofilariasis in the eyelid caused by Dirofilaria repens: An imported case.

Archivos de la Sociedad Espanola de Oftalmologia·2016

Area of Science:

  • Molecular Biology
  • Parasitology
  • Genetics

Background:

  • Trichinella parasites pose a significant public health concern.
  • Accurate identification of Trichinella species is crucial for epidemiological studies and control strategies.
  • Previous studies have utilized DNA-based methods for Trichinella characterization.

Purpose of the Study:

  • To characterize Spanish Trichinella isolates using DNA analysis.
  • To investigate the genetic relatedness and potential for further subdivision within these isolates.

Main Methods:

  • Genomic DNA was extracted from 13 Spanish Trichinella isolates.
  • DNA cross-hybridisation tests were performed to assess genetic similarity.
  • Restriction-fragment polymorphism analysis was conducted using a T. spiralis DNA repetitive fragment probe.

Related Experiment Videos

Main Results:

  • Genomic DNA cross-hybridisation identified two distinct groups among the isolates.
  • These two groups showed only weak cross-reactions, suggesting some genetic divergence.
  • Restriction-fragment polymorphism analysis failed to reveal significant differences between the two identified groups.

Conclusions:

  • The study identified two genetically distinct groups within the Spanish Trichinella isolates based on DNA cross-hybridisation.
  • Despite initial differentiation, further analysis using RFLP did not provide finer resolution, suggesting limited genetic variability detectable by this method.
  • These findings contribute to understanding the genetic landscape of Trichinella in Spain.