Chloride determination in serum by a flow-injection analysis precipitation pseudo-titration technique using a...

Area of Science:

• Analytical Chemistry
• Electrochemistry
• Clinical Diagnostics

Background:

• Flow-injection analysis (FIA) is a widely used technique for rapid chemical analysis.
• Accurate determination of chloride in serum is crucial for diagnosing various medical conditions.
• Existing methods for chloride determination may require complex instrumentation or procedures.

Purpose of the Study:

• To describe a simply constructed, tubular, all-solid-state, flow-through silver electrode for FIA.
• To present a novel FIA method for determining chloride in serum.
• To evaluate the performance of the developed silver electrode and FIA technique.

Main Methods:

• Fabrication of a tubular, all-solid-state, flow-through silver electrode.
• Development of a single-line manifold for FIA incorporating the silver electrode.
• Utilizing a low concentration of silver ion (5 x 10(-4) M) in the carrier stream.
• Employing a precipitation pseudo-titration FIA technique for chloride determination.

Main Results:

• The developed silver electrode is simple to construct and functions effectively in a flow-through configuration.
• The FIA method using the silver electrode allows for accurate determination of chloride in serum.
• The technique achieves a sampling frequency of approximately 60 samples per hour.

Conclusions:

• The described tubular, all-solid-state, flow-through silver electrode is a practical tool for FIA.
• The precipitation pseudo-titration FIA technique with this electrode offers a reliable and efficient method for serum chloride analysis.
• This approach provides a valuable alternative for routine clinical laboratory testing.