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Updated: Aug 6, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

Fast pre-column derivatization of aminoglycosides with 1-fluoro-2,4-dinitrobenzene and its application to

D M Barends1, J C Brouwers, A Hulshoff

  • 1Department of Chemical Pharmacy, University of Utrecht, Pharmaceutical Laboratory, Catharijnesingel 60, 3511 GH Utrecht, The Netherlands.

Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1987
PubMed
Abstract

No abstract available in PubMed .

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Preparation of 1° Amines: Gabriel Synthesis01:28

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Direct alkylation is not a suitable method for synthesizing amines because it produces polyalkylated products. Gabriel synthesis is the most preferred method to exclusively make primary amines. The method uses phthalimide, which contains a protected form of nitrogen that participates in alkylation only once to predominantly give primary amines.
Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...