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Enzymatic determination of total cholesterol in serum by flow injection analysis
J M Fernandez-Romero1, M D Luque de Castro, M Valcarcel
1Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Córdoba, Spain.
Abstract:
Several methods are proposed for the enzymatic determination of total cholesterol in serum by flow injection analysis (FIA). The use of the merging-zones mode, with or without halting of the reacting plug in the photometric or fluorimetric detector, has allowed the development of four precise methods for the determination of this analyte; calibration curves were linear for ranges of 0.02-0.2 and 0.005-0.05 gl(-1). These methods have been applied to the determination of cholesterol in serum with excellent results.
