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Updated: Aug 6, 2026

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
07:31

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

Ion-pair liquid chromatographic assay of angiotensin-converting enzyme activity

V Maurich1, A Pitotti, L Vio

  • 1Istituto di Tecnica Farmaceutica, Università di Trieste, 34100 Trieste, Italy.

Journal of Pharmaceutical and Biomedical Analysis
|January 1, 1985
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.