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Simple and rapid high-performance liquid chromatographic assay for esmolol
Journal of Chromatography
|September 18, 1991
Summary
This study details a new method for measuring esmolol concentrations in blood using high-performance liquid chromatography. The validated assay offers accurate and reliable esmolol quantification for pharmacokinetic studies.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Biochemistry
Background:
- Accurate quantification of drugs in biological matrices is crucial for pharmacokinetic studies.
- Esmolol, a short-acting beta-blocker, requires precise measurement in blood for therapeutic monitoring.
Purpose of the Study:
- To develop and validate a sensitive and reliable method for determining esmolol concentrations in human blood.
- To establish an assay suitable for pharmacokinetic and pharmacodynamic investigations of esmolol.
Main Methods:
- Esmolol extraction from blood using dichloromethane to inhibit esterase activity.
- Quantification by high-performance liquid chromatography (HPLC) with 3-methoxy-O-demethylencainide as the internal standard.
Main Results:
- The assay demonstrated a limit of detection of 5 ng/ml.
- Linearity was observed between 10-30,000 ng/ml.
- Mean recoveries were 84% (absolute) and 89% (relative) with CVs <3%.
Conclusions:
- The described HPLC method provides a robust and accurate means for quantifying esmolol in blood.
- This validated assay is suitable for use in pharmacokinetic and pharmacodynamic studies.