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Updated: Aug 6, 2026
Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
[Determination of human serum glutathione peroxidase activity, by using hydrogen peroxide and
Abstract:
The method has been developed for the assay of human serum glutathione:H2O2-oxidoreductase activity. The reaction medium comprises tris-HCl buffer (0.04 M; pH 8.5), 4.4 mM EDTA, 10 mM NaN3, 1 mM GSH, 400 microM Y2O2. The incubation time of 60 seconds at 37 degrees C was chosen. There was a decrease in GSH in the reaction with 5,5'-dithio-bis(2-nitrobenzoic acid). This method is recommended for the application to the assay of selenium-dependent glutathione peroxidase because hydrogen peroxide (H2O2) used in this method is a more specific substrate than commonly used organic hydroperoxides.
