Genetic analysis and gene mapping of purple stigma in rice

A new double-haploid (rdh) rice plant with purple stigma and red seeds was discovered by tissue culture. Genetic analysis suggested that the trait of rdh purple stigma was controlled by a pair of dominant gene. Polymorphic analysis of microsatellite markers demonstrated that the purple stigma gene of rdh was located on rice chromosome 6 at 4.2 cM, 0.35 cM and 0.53 cM from microsatellite markers RM276, RM253 and RM111, respectively. It was believed that the purple stigma gene of rdh was the first mapped purple stigma gene on rice chromosome 6. This purple stigma gene was designated tentatively as Ps-4.