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Updated: Aug 6, 2026

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
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Biological role of the CXCR4-SDF-1 axis in normal human hematopoietic cells

Marcin Majka1, Mariusz Z Ratajczak

  • 1James Graham Brown Cancer Center, University of Louisville, KY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 2, 2006
PubMed

Stromal-derived factor (SDF)-1, an alpha-chemokine that binds to G protein-coupled seven transmembrane-spanning receptor, CXCR4, plays an important and unique role in regulating the trafficking of normal hematopoietic stem/progenitor cells and their homing/retention in bone marrow. The same axis also modulates several biological processes in more differentiated cells from the granulocyte-monocytic, erythroid, and megakaryocytic lineages. In this chapter, experimental details are described for the isolation of early human hematopoietic cells, such as CD34+ mononuclear cells, myeloblasts, erythroblasts, and megakaryoblasts. These cells can be used routinely for studying the role of the CXCR4-SDF-1 axis in normal human hematopoiesis.

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