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Updated: Jul 29, 2026
09:56
Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
Published on: August 21, 2019
Extended focus depth for Fourier domain optical coherence microscopy.
R A Leitgeb1, M Villiger, A H Bachmann
1Laboratoire d'Optique Biomedicale, Ecole Polytechnique Federale de Lausanne, CH-1015 Lausanne, Switzerland. rainerleitgeb@epfl.ch
Optics Letters
|August 2, 2006
Summary
We developed a new Fourier domain optical coherence microscopy detection scheme. It achieves high transverse resolution over an extended focal range, improving imaging capabilities.
Area of Science:
- Biomedical optics
- Microscopy techniques
- Optical imaging
Background:
- Fourier domain optical coherence microscopy (FD-OCM) is a powerful imaging technique.
- Achieving high transverse resolution over an extended axial range remains a challenge in FD-OCM.
Purpose of the Study:
- To introduce and validate a novel detection scheme for FD-OCM.
- To enhance transverse resolution and extend the usable focal range in FD-OCM.
Main Methods:
- Implementation of a new detection scheme within the Fourier domain optical coherence microscopy framework.
- Utilizing a broad-bandwidth Ti:sapphire laser source.
- Experimental verification of resolution and sensitivity.
Main Results:
- Achieved nearly constant transverse resolution of approximately 1.5 micrometers.
- Demonstrated this resolution over an axially extended focal range of 200 micrometers.
- Verified a maximum sensitivity of 105 dB and an axial resolution of 3 micrometers in air.
Conclusions:
- The novel detection scheme significantly improves transverse resolution in FD-OCM.
- The extended focal range broadens the applicability of FD-OCM for various biological samples.
- This advancement offers enhanced capabilities for high-resolution microscopic imaging.
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