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Updated: Aug 6, 2026
09:03
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Published on: May 29, 2014
Isolation and characterization of human embryonic stem cells
Maisam Mitalipova1, Gail Palmarini
1Department of Animal and Dairy Sciences, University of Georgia, Athens, USA.
Methods in Molecular Biology (Clifton, N.J.)
|August 3, 2006
Summary
This study provides detailed protocols for deriving and maintaining human embryonic stem (hES) cells. These guidelines simplify complex procedures for researchers establishing hES cell laboratories.
Area of Science:
- * Stem Cell Biology
- * Developmental Biology
- * Reproductive Medicine
Background:
- * Deriving human embryonic stem (hES) cells presents significant challenges.
- * Current isolation and maintenance methods are visually and manually complex.
- * Procedures often involve mechanical or enzymatic passaging using collagenase or trypsin.
Purpose of the Study:
- * To describe detailed protocols for hES cell derivation and maintenance.
- * To outline methods for characterizing hES cells in vitro.
- * To present protocols for assessing hES cell differentiation potential in vivo.
Main Methods:
- * Detailed step-by-step protocols for hES cell derivation.
- * Established methods for in vitro maintenance and characterization of hES cells.
- * Protocols for in vivo differentiation potential testing.
Main Results:
- * Provided comprehensive guidelines for hES cell laboratory setup.
- * Demonstrated successful derivation and maintenance protocols.
- * Characterization and differentiation protocols were detailed.
Conclusions:
- * The described protocols simplify complex hES cell procedures.
- * These guidelines will assist researchers in establishing functional hES cell laboratories.
- * Facilitates reproducible research in human embryonic stem cell biology.
Related Concept Videos
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.