Human Secretomes, Plant Exosomes, and Hybrid Secretomes Intradermal Injections for Hair Regeneration: Experimental...

Area of Science:

• Regenerative Medicine
• Dermatology
• Cell Biology

Background:

• Hair follicle regeneration relies on complex cellular interactions and signaling pathways.
• Injectable biologics like human secretomes and plant exosomes are emerging therapeutics for hair loss.
• Their efficacy in stimulating hair growth in intact skin requires further investigation.

Purpose of the Study:

• To evaluate and compare the effects of human adipose-derived secretomes, plant-derived exosomes, and a hybrid formulation on hair regeneration.
• To assess the impact of these formulations on key markers of hair follicle activity in vivo.

Main Methods:

• A randomized, blinded, controlled study was performed on healthy hamsters.
• Animals received intradermal injections of human secretomes, plant exosomes, hybrid secretomes, or saline.
• Skin biopsies were analyzed using histology and immunohistochemistry for proliferation (Ki67), Wnt activation (β-catenin), and stem cell markers (CD34).

Main Results:

• Hybrid secretomes demonstrated the highest levels of Ki67-positive cells, indicating increased proliferation.
• Human secretomes showed the most pronounced nuclear β-catenin localization, suggesting Wnt pathway activation.
• CD34 expression, a stem cell marker, was highest in hybrid secretomes, followed by human secretomes.

Conclusions:

• Hybrid secretomes effectively promoted proliferative and stem cell-associated markers for hair regeneration.
• Human secretomes significantly activated the Wnt/β-catenin pathway.
• These findings highlight the distinct mechanisms of action for different exosome-based formulations in hair follicle regeneration.