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Published on: January 14, 2017
Inhibition of Bacillus licheniformis LMG 19409 from ropy cider by enterocin AS-48
M J Grande1, R Lucas, H Abriouel
1Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain.
Aims:
To determine the activity of enterocin AS-48 against ropy-forming Bacillus licheniformis from cider.
Methods And Results:
Enterocin AS-48 was tested on B. licheniformis LMG 19409 from ropy cider in MRS-G broth, fresh-made apple juice and in two commercial apple ciders (A and B). Bacillus licheniformis was rapidly inactivated in MRS-G by 0.5 microg ml(-1)AS-48 and in fresh-made apple juice by 3 microg ml(-1). Concentration-dependent inactivation of this bacterium in two commercial apple ciders (A and B) stored at 4, 15 and 30 degrees C for 15 days was also demonstrated. Counts from heat-activated endospores in cider A plus AS-48 decreased very slowly. Application of combined treatments of heat (95 degrees C) and enterocin AS-48 reduced the time required to achieved complete inactivation of intact spores in cider A to 4 min for 6 microg ml(-1) and to 1 min for 12 microg ml(-1). D and z values also decreased as the bacteriocin concentration increased.
Conclusion:
Enterocin AS-48 can inhibit ropy-forming B. licheniformis in apple cider and increase the heat sensitivity of spores.
Significance And Impact Of The Study:
Results from this study support the potential use of enterocin AS-48 to control B. licheniformis in apple cider.
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