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Bacterial Immobilization for Imaging by Atomic Force Microscopy
Published on: August 10, 2011
Atomic force microscopy of a hydrated bacterial surface protein
W Wiegräbe1, M Nonnenmacher, R Guckenberger
1Max-Planck Institut für Biochemie, Martinsried, Germany.
Journal of Microscopy
|July 1, 1991
Abstract:
The protein surface layer of the bacterium Deinococcus radiodurans (HPI layer) was examined with an atomic force microscope (AFM). The measurements on the air-dried, but still hydrated layer were performed in the attractive imaging mode in which the forces between tip and sample are much smaller than in AFM in the repulsive mode or in scanning tunnelling microscopy (STM). The results are compared with STM and transmission electron microscopy (TEM) data.

