Related Experiment Video

Updated: Aug 6, 2026

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
10:14

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Published on: May 26, 2023

[Pathomorphology of membranes appearing in proliferative vitreoretinopathies]

Iwona Laudańska-Olszewska1, Wojciech Omulecki, Krzysztof Dziegielewski

  • 1Kliniki Chorób Oczu Katedry Chorób Oczu Uniwersytetu Medycznego w Lodzi.

Klinika Oczna
|August 4, 2006
PubMed

Insights

This study identified retinal pigment epithelial cells, glial cells, macrophages, and T lymphocytes in epiretinal membranes from proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR). These cells may play a role in forming vitreoretinal membranes in these conditions.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Immunohistochemistry

Context:

  • Epiretinal membranes are a hallmark of proliferative vitreoretinopathy (PVR) and proliferative diabetic retinopathy (PDR).
  • Understanding the cellular composition of these membranes is crucial for elucidating disease pathogenesis.

Purpose:

  • To investigate the cellular and extracellular matrix components of epiretinal membranes in PVR and PDR using immunohistochemical techniques.
  • To identify specific cell types and their potential contribution to membrane formation.

Summary:

  • Immunohistochemistry was employed to analyze ten epiretinal membranes from PVR and five from PDR surgical specimens.
  • Retinal pigment epithelial cells, glial cells, macrophages, T lymphocytes, and type IV collagen were identified within these membranes.
  • The presence of these cells suggests their active involvement in the development of vitreoretinal membranes in PVR and PDR.

Impact:

  • Provides insights into the cellular mechanisms underlying PVR and PDR progression.
  • Highlights potential cellular targets for future therapeutic interventions.
  • Contributes to the understanding of vitreoretinal membrane formation in complex ocular diseases.

More Related Videos

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies12:28

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Published on: March 12, 2022

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes10:46

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Published on: October 7, 2018

Related Concept Videos

Diabetic Retinopathy01:27

Diabetic Retinopathy

DefinitionDiabetic retinopathy is a microvascular complication of diabetes affecting the retinal blood vessels.Risk FactorsDiabetic retinopathy is present in almost all individuals with type 1 diabetes and more than 60% of those with type 2 diabetes after two decades of disease.The risk increases with poor glycemic control, hypertension, dyslipidemia, smoking, pregnancy, and puberty.Although cataracts and glaucoma are also more frequent in people with diabetes, retinopathy remains the leading...
Types of Membrane Protrusions01:28

Types of Membrane Protrusions

The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most  widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
The microvilli, an example of stable protrusions, are finger-like projections with a...
Fluid Mosaic Model01:19

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich with the analogy of...
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Membrane Domains01:18

Membrane Domains

The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...