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Related Experiment Videos

Structure of the gene for human complement protein decay accelerating factor.

T W Post1, M A Arce, M K Liszewski

  • 1Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.

Journal of Immunology (Baltimore, Md. : 1950)
|January 15, 1990
PubMed
Summary

The human Decay Accelerating Factor (DAF) gene, part of the RCA family, spans 40 kb with 11 exons and varying intron lengths. Its exon-intron structure aids in understanding DAF protein domain functions.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Immunology

Background:

  • Decay Accelerating Factor (DAF) is a membrane protein in the regulators of complement activation (RCA) gene family.
  • DAF proteins contain multiple copies of a short consensus repeat (SCR) domain.
  • The RCA gene family shares common evolutionary heritage.

Purpose of the Study:

  • To identify and characterize the genomic structure of the human DAF gene.
  • To analyze the exon-intron organization of the DAF gene.
  • To provide insights into the functional domains of DAF.

Main Methods:

  • A DAF cDNA clone was used to identify overlapping bacteriophage genomic clones.
  • The human DAF gene was analyzed for its span, number of exons, and intron lengths.

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  • Exon and intron boundaries were determined, including splice junctions.

    • Main Results:

    • The human DAF gene spans approximately 40 kb and comprises 11 exons with significant variation in exon and intron lengths.
    • Specific SCR domains (I, II, IV) are encoded by single exons, while SCR III is split across two exons.
    • A serine/threonine-rich domain is encoded by three exons, and an Alu family sequence is present in exon 10, indicating alternative splicing.

    Conclusions:

    • The exon-intron structure of the DAF gene provides a framework for understanding the functional roles of its various domains.
    • The gene's organization supports the common evolutionary origin of RCA family members.
    • Further research into DAF's domain functions can be facilitated by this detailed genomic analysis.