A microRNA detection system based on padlock probes and rolling circle amplification
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Updated: Aug 6, 2026
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Søren Peter Jonstrup1, Jørn Koch, Jørgen Kjems
1Department of Molecular Biology, University of Aarhus, C.F. Møllers Allé, DK-8000 Arhus C, Denmark.
Abstract:
The differential expression and the regulatory roles of microRNAs (miRNAs) are being studied intensively these years. Their minute size of only 19-24 nucleotides and strong sequence similarity among related species call for enhanced methods for reliable detection and quantification. Moreover, miRNA expression is generally restricted to a limited number of specific cells within an organism and therefore requires highly sensitive detection methods. Here we present a simple and reliable miRNA detection protocol based on padlock probes and rolling circle amplification. It can be performed without specialized equipment and is capable of measuring the content of specific miRNAs in a few nanograms of total RNA.
