Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The A-minor motifs in the decoding recognition process.

A Lescoute1, E Westhof

  • 1Institut de biologie moleculaire et cellulaire du CNRS, UPR 'Architecture et Réactivité de l'ARN', universite Louis-Pasteur, 15, rue R.-Descartes, 67084 Strasbourg, France.

Biochimie
|August 8, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Delayed elimination of methotrexate associated with co-administration of proton pump inhibitors.

Anticancer research·2010
Same author

Large telomerase RNA, telomere length heterogeneity and escape from senescence in Candida glabrata.

FEBS letters·2009
Same author

Correlated motions in DNA.

Biophysical journal·2009
Same author

The interaction networks of structured RNAs.

Nucleic acids research·2006
Same author

A molecular dynamics simulation study of an aminoglycoside/A-site RNA complex: conformational and hydration patterns.

Biochimie·2006
Same author

Mutagenesis of 16S rRNA C1409-G1491 base-pair differentiates between 6'OH and 6'NH3+ aminoglycosides.

Journal of molecular biology·2005
Same journal

Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

Biochimie·2026
Same journal

Renal failure-driven luminal ammonia production impairs gut barrier function in CKD.

Biochimie·2026
Same journal

Conditional Knockout of Indoleamine 2, 3-Dioxygenase-1 in Osteoprogenitor Cells in Mice Results in Sex-dependent Differences in Bone Mass.

Biochimie·2026
Same journal

Sedentariness disrupts, while exercise restores, thermogenic and metabolic plasticity in inguinal adipose tissue of mice.

Biochimie·2026
Same journal

Chrononutrition as a modulator of retinal metabolic resilience: A translational framework linking circadian biology to ocular disease.

Biochimie·2026
Same journal

Heterologous expression, purification, and biophysical characterisation of the cobalt-dependent nitrile hydratase from Rhodococcus rhodochrous ATCC BAA-870.

Biochimie·2026
See all related articles

Adenines in the ribosomal A site dynamically shift, enabling accurate codon reading. Aminoglycoside antibiotics freeze this movement, disrupting protein synthesis regulation.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • The ribosomal A site is crucial for decoding genetic information.
  • Adenine bases (A1492, A1493) within the A site exhibit dynamic conformational changes.
  • These dynamics are essential for accurate codon:anticodon recognition during translation.

Purpose of the Study:

  • To describe the formation of A-minor motifs involving adenine binding.
  • To review the conformations of the bacterial ribosomal A site in various crystal structures.
  • To elucidate the mechanism by which aminoglycoside antibiotics affect A site conformation and function.

Main Methods:

  • Analysis of crystal structures of the bacterial ribosomal A site.
  • Structural comparison of A site conformations in different functional states.

Related Experiment Videos

  • Investigation of adenine base interactions within the minor groove of Watson-Crick base pairs.

    • Main Results:

    • A-minor motifs form through adenine binding into the minor groove of stacked base pairs.
    • Adenines A1492 and A1493 exist in equilibrium between tucked-in and bulged-out conformations.
    • Aminoglycoside antibiotics stabilize the bulged-out conformation, disrupting A site regulation.

    Conclusions:

    • The dynamic equilibrium of adenines in the A site is vital for precise decoding.
    • Aminoglycoside antibiotics act by locking the A site into a non-functional conformation.
    • This mechanism highlights a key target for antibiotic intervention in bacterial translation.