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Related Experiment Videos

Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection.

S Piermarini1, L Micheli, N H S Ammida

  • 1Dipartimento di Scienze e Tecnologie Chimiche, Università Tor Vergata, via della Ricerca Scientifica, 00133 Rome, Italy.

Biosensors & Bioelectronics
|August 9, 2006
PubMed
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A novel electrochemical immunosensor array effectively detects aflatoxin B1 (AFB1) in corn. This sensitive method offers accurate quantification for food safety, with a detection limit of 30 pg/mL.

Area of Science:

  • Analytical Chemistry
  • Biosensor Technology
  • Food Safety Analysis

Background:

  • Aflatoxin B1 (AFB1) is a potent carcinogen commonly found in food commodities.
  • Accurate and sensitive detection methods are crucial for ensuring food safety and preventing mycotoxin contamination.
  • Existing detection methods may require complex procedures or expensive equipment.

Purpose of the Study:

  • To develop and validate a novel analytical immunosensor array for the sensitive detection of AFB1.
  • To evaluate the electrochemical performance and analytical characteristics of the proposed system.
  • To assess the applicability of the immunosensor for AFB1 determination in real food samples, such as corn.

Main Methods:

  • Development of a microtiter plate coupled to a multichannel electrochemical detection (MED) system.

Related Experiment Videos

  • Utilizing intermittent pulse amperometry (IPA) technique with screen-printed electrodes.
  • Modification of a 96-well screen-printed microplate for a competitive indirect enzyme-linked immunoassay (ELISA) format.
  • Analysis of corn samples spiked with AFB1 to evaluate extraction efficiency and matrix effects.

    • Main Results:

    • The immunosensor array demonstrated a low limit of detection (LOD) of 30 pg/mL for AFB1.
    • A working range of 0.05–2 ng/mL was established with good reproducibility.
    • High recovery rates (103 ± 8%) were achieved in spiked corn samples, indicating minimal matrix effects.
    • The assay showed good specificity, distinguishing AFB1 from most other aflatoxins, except AFG1.

    Conclusions:

    • The proposed electrochemical immunosensor array is a suitable tool for accurate and sensitive AFB1 determination in corn.
    • The system offers a promising alternative for routine food safety monitoring and quality control.
    • Further optimization could enhance specificity towards AFG1 for comprehensive aflatoxin profiling.