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MetaVision-SPR: a 96-well chromatic Meta-SPR imaging platform for tumor biomarker quantification and apparent kinetic
Liangjun Yang1, Chuncan Zhou1, Mingqian Chen2
1Department of Nano Biosensing and Artificial Intelligence, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Abstract:
We report MetaVision-SPR, a 96-well chromatic metasurface plasmon resonance (Meta-SPR) imaging platform for serum biomarker quantification and apparent kinetic analysis. The system integrates a nanocup-array Meta-SPR chip, light-emitting diode (LED) illumination, an industrial camera, and computational correction including automated well recognition, sucrose-reference-based optical flat-field correction, and Δ(R-G) chromatic readout. Spectral simulation and optical response matching identified the 20/75/25 nm Ti/Ag/Au structure and Δ(R-G) signal as the optimal chip-readout combination for quantitative imaging. Flat-field correction reduced inter-well variation in a Protein A/immunoglobulin G (IgG) assay from 26.74% to 6.74%, while programmable shaking improved mass transport in the open-well format. Using gold nanoparticle (AuNP)-enhanced sandwich assays, the platform enabled sensitive detection of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) with limits of detection (LoDs) of 5.0 pg/mL for AFP, 10.0 pg/mL for CEA and 0.49 U/mL for CA19-9. Clinical serum measurements showed reference-method consistency with hospital electrochemiluminescence immunoassay (ECLIA) and commercial enzyme-linked immunosorbent assay (ELISA) results. In addition, the system supported real-time measurement of binding curves for antibody-drug conjugate and lipopolysaccharide-related interactions, yielding apparent kinetic parameters suitable for high-throughput screening. These results support MetaVision-SPR as a low-hardware-cost 96-well chromatic Meta-SPR workflow for serum biomarker measurement and apparent interaction ranking.
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