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Updated: Aug 6, 2026
04:58
Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds
Published on: October 20, 2012
Mediator responses in surgical infections
1Rikshospitalet University Hospital, Institute for Surgical Research, University of Oslo, Oslo, Norway. a.o.aasen@rh.uio.no
Surgical Infections
|August 10, 2006
Summary
Sepsis involves mediator release, activating plasma cascades and cytokines, leading to organ dysfunction. Early detection of lipopolysaccharide (LPS) and mediators aids in monitoring infection progression.
Area of Science:
- Biochemistry
- Immunology
- Pathophysiology
Background:
- Sepsis involves extensive mediator release, impacting morbidity and mortality.
- Understanding these mediators is crucial for developing effective therapeutics for surgical infections.
Purpose of the Study:
- To investigate the role of mediators like lipopolysaccharide (LPS) and peptidoglycan in surgical infections.
- To explore the activation of plasma cascade systems and cytokine release.
- To establish a monitoring system for early infection detection.
Main Methods:
- In vitro studies exposing human plasma to LPS and whole blood to peptidoglycan.
- In vivo studies using rat, dog, and pig models with LPS and peptidoglycan.
- Clinical study correlating serum LPS with multiple organ dysfunction in ICU patients.
Main Results:
- LPS exposure activated plasma kallikrein-kinin system, coagulation, fibrinolysis, and complement cascades.
- Peptidoglycan induced cytokine release, organ dysfunction, and matrix metalloproteinase-9 (MMP-9) activity.
- Clinical findings linked LPS to multiple organ dysfunction and failure, with activated plasma cascades and cytokine release.
Conclusions:
- These findings support the development of a monitoring system for early infection recognition.
- The study highlights the systemic inflammatory response in sepsis.
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