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Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
West Nile virus in mosquitoes of northern Ohio, 2003
Bradley J White1, David R Andrew, Nicole Z Mans
1Department of Biological Sciences, University of Notre Dame, Indiana 46556, USA.
Abstract:
From June 19, 2003 to August 18, 2003, we surveyed the mosquitoes of Oberlin, OH, for West Nile Virus (WNV) infection using reverse transcriptase-polymerase chain reaction. A total of 12,055 mosquitoes, representing 17 species or species groups and 4 genera, were collected in gravid traps at seven sites throughout the city, with Culex pipiens/restuans being the most abundant and showing the highest minimum infection rate (MIR) of 0.78. This represents a decrease in WNV enzootic activity from the previous year. Both Cx. pipiens/restuans abundance and MIR increased significantly with date. However, we found no correlation between Cx. pipiens/restuans abundance and MIR.
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