Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Circumventing air bubbles in microfluidic systems and quantitative continuous-flow PCR applications.

Tsuyoshi Nakayama1, Yasunori Kurosawa, Satoshi Furui

  • 1School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.

Analytical and Bioanalytical Chemistry
|August 10, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prophylactic percutaneous intra-aortic balloon occlusion reduces blood loss during anterior acetabular fracture surgery: a propensity score-matched study.

Archives of orthopaedic and trauma surgery·2026
Same author

Recent advances towards BACE1 drug discovery and therapeutics design.

RSC medicinal chemistry·2026
Same author

Bacterial imprinted polymer-based detection of <i>Escherichia coli</i> using polydopamine on gold nanodendrite/graphene oxide modified electrodes.

The Analyst·2026
Same author

Recent progress in wearable electrochemical sensors based on MXene-conductive hydrogels.

The Analyst·2026
Same author

Intraoperative fluoroscopic evaluation of trochanteric fracture reduction using a novel anteromedial cortex view: A multicenter prospective observational study.

Injury·2026
Same author

A tri-responsive sensor based on co-encapsulated organic probes and multifunctional bimetallic V/Ce-MOF nanozyme in a hydrogel for the detection of l-Serine in saliva.

Talanta·2026

This study introduces a novel liquid-flow method using fluorinated oil to prevent air bubbles in microfluidic devices for polymerase chain reaction (PCR). This innovation enables stable, continuous-flow PCR for accurate DNA quantification in micro-total analysis systems.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Genomics

Background:

  • Polymerase chain reaction (PCR) is crucial for genomics and cell analysis.
  • Integrating PCR into microfluidic systems (micro-total analysis systems, muTAS) is vital but challenged by air bubble formation.
  • Unstable flow due to air bubbles hinders reliable high-temperature reactions in microchannels.

Purpose of the Study:

  • To develop a novel liquid-flow method to overcome air bubble generation issues in microfluidic PCR.
  • To enable stable and continuous-flow polymerase chain reaction (PCR) on a microfluidic device.
  • To validate the device's accuracy for quantitative DNA analysis, essential for muTAS integration.

Main Methods:

  • Fabrication of a polydimethylsiloxane (PDMS)-based microfluidic device using soft-lithography.

Related Experiment Videos

  • Introduction of fluorinated oil as a capping liquid to prevent air bubbles before sample introduction.
  • Application of the device for continuous-flow PCR, including quantitative PCR with TaqMan technology and laser detection.

    • Main Results:

    • The novel liquid-flow method successfully prevented air bubble generation, ensuring stable flow.
    • Continuous-flow PCR was achieved on-chip, demonstrating the device's capability for high-temperature reactions.
    • Quantitative PCR results showed a linear relationship between threshold cycle (Ct) and initial DNA concentration, enabling accurate DNA quantification.

    Conclusions:

    • The developed microfluidic device with a novel liquid-flow method effectively addresses air bubble challenges in PCR.
    • The device facilitates accurate quantitative DNA analysis, crucial for integrating PCR into micro-total analysis systems (muTAS).
    • This technology shows significant potential for diverse research applications requiring precise DNA quantification in microfluidic platforms.