Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A control model for tibial cortex neovascularization in the bone chamber.

H Winet1, J Y Bao, R Moffat

  • 1Department of Orthopedics, University of Southern California, Los Angeles.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|January 1, 1990
PubMed
Summary

Neovascularization precedes bone formation in rabbit tibias, with vessel growth adapting to tissue type. Blood flow doesn't always predict oxygen supply in healing bone.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The AADOCR Position Statement on Topical Fluoride.

Journal of dental research·2025
Same author

The AADOCR Position Statement on Community Water Fluoridation.

Journal of dental research·2025
Same author

Inhibiting responses under the watch of a recently synchronized peer increases self-monitoring: evidence from functional near-infrared spectroscopy.

Open biology·2024
Same author

[Efficacy of 0.05% cyclosporine A combined with vitamin A palmitate in the treatment of meibomian gland dysfunction-related dry eye].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2024
Same author

In infertile women with subclinical hypothyroidism, with or without thyroid peroxidase antibodies, serum TSH during pregnancy follows preconception values and thyroid hormones remain stable.

Human reproduction open·2023
Same author

[Clinical features and predictors of early neurological deterioration in acute isolated pontine infarction].

Zhonghua yi xue za zhi·2023

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Vascular Biology

Background:

  • Understanding bone healing requires insights into vascularization processes.
  • The optical bone chamber implant (BCI) allows in vivo monitoring of tissue regeneration.
  • Neovascularization and osteogenesis dynamics in cortical defects remain areas for detailed study.

Purpose of the Study:

  • To investigate the spatiotemporal dynamics of neovascularization and bone apposition in a rabbit tibial defect model.
  • To quantify vessel characteristics, including length per unit volume (L/V), caliber (C), and flow velocity (u), during bone healing.
  • To elucidate the relationship between blood supply and nutrient exchange in newly forming bone.

Main Methods:

  • Utilized the optical bone chamber implant (BCI) in rabbits to visualize tibial cortical defects.

Related Experiment Videos

  • Employed intravital and fluorescence microscopy with FITC-dextran to image vasculature over 3-8 weeks postimplantation.
  • Applied digital image processing to analyze videotaped observations, measuring L/V, C, and u.

    • Main Results:

    • Neovascularization was observed to precede new bone formation (neo-osteogenesis).
    • Vessel length per unit volume (L/V) increased in trabeculae over time but remained constant in fibrous tissue.
    • Blood supply (Q) and nutrient exchange area were not consistently correlated in healing trabeculae.

    Conclusions:

    • Neovascularization is a prerequisite for osteogenesis in this model.
    • Vessel alignment tends to follow the tibial axis.
    • Constant L/V in fibrous tissue suggests angiogenesis balances vessel destruction, while increasing L/V in trabeculae indicates ongoing vascular development.