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Updated: Aug 6, 2026

Quantitation of Endothelial Cell Adhesiveness In Vitro
Published on: June 18, 2015
[Hemangiopoiet in modulates adhesive properties of endothelial cells]
Xiao-Jing Wang1, Yong-Jun Liu, Shi-Hong Lu
1State Key Laboratory of Experimental Hematology, Tianjin Economic-Technological Development Area Life and Technology Research Center, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
Objective:
To explore the effect of hemangiopoietin (HAPO) on the adhesive properties of human umbilical vein endothelial cells (HUVEC).
Methods:
The adhesion of HUVEC and the expressions of CD54, CD102, CD106, CD31, CD62E, and CD62P were measured by adhesion assay, flow cytometry, and semi-quantitative RT-PCR.
Results:
HAPO enhanced the total adherence of HUVEC in a concentration-dependent manner. Flow cytometry analysis revealed that the treatment of HAPO resulted in a significantly increased expression of CD106 and CD62E on HUVEC in a time-dependent manner. When HUVEC were incubated with HAPO for 6 h, the percentage of CD106 + HUVEC and CD62E HUVEC increased about 2.10 folds and 5.84 folds, respectively, compared with control. The time-course of adhesive molecules mRNA expression indicated that the expression of CD106 and CD62E reached at the maximum 1.86 folds and 6.16 folds, respectively, compared with control.
Conclusion:
HAPO may facilitate the homing of hematopoietic stem/progenitor cells.
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