Related Experiment Video

Updated: Mar 2, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.4K

Resveratrol inactivates PI3K/Akt signaling through upregulating BMP7 in human colon cancer cells

Yu-Hua Zeng1, Lin-Yun Zhou1, Qian-Zhao Chen1

  • 1Department of Pharmacology, School of Pharmacy, Chongqing Medical University, Chongqing 400016, P.R. China.

Oncology Reports
|May 24, 2017
PubMed

Insights

Resveratrol shows anticancer effects in colon cancer cells by upregulating BMP7. This mechanism involves inactivating the PI3K/Akt pathway, suggesting Resveratrol as a potential colon cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Colon cancer presents a significant global health burden with ongoing challenges in treatment and diagnosis.
  • Resveratrol (Res) has emerged as a potential therapeutic agent for cancer, but its precise mechanisms remain largely unelucidated.
  • Understanding the molecular pathways influenced by Resveratrol is crucial for developing effective colon cancer therapies.

Purpose of the Study:

  • To investigate the anticancer activity of Resveratrol in human colon cancer cells (HCT116).
  • To elucidate the underlying molecular mechanism of Resveratrol's anti-colon cancer effects.
  • To explore the role of Bone Morphogenetic Protein 7 (BMP7) in mediating Resveratrol's activity.

Main Methods:

  • Cell viability assays to assess anticancer effects.
  • Flow cytometry for cell cycle analysis.
  • Polymerase Chain Reaction (PCR) and Western blot analysis to evaluate gene and protein expression and phosphorylation.
  • Experiments involving exogenous BMP7 and BMP7-specific antibodies to confirm pathway involvement.

Main Results:

  • Resveratrol demonstrated significant anticancer activity in HCT116 colon cancer cells.
  • Resveratrol treatment led to a notable upregulation of BMP7 expression in HCT116 cells.
  • The anticancer effects of Resveratrol were enhanced by exogenous BMP7 and diminished by BMP7 inhibition.
  • Resveratrol inhibited the phosphorylation of Akt1/2/3 and PTEN, key components of the PI3K/Akt signaling pathway.
  • BMP7 upregulation by Resveratrol was found to be critical for the observed inactivation of the PI3K/Akt pathway.

Conclusions:

  • Resveratrol exhibits promising anticancer properties against colon cancer cells.
  • The mechanism involves the upregulation of BMP7, which subsequently inactivates the PI3K/Akt signaling pathway.
  • Resveratrol represents a potential therapeutic candidate for colon cancer, warranting further clinical investigation.

More Related Videos

An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation08:48

An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation

Published on: June 30, 2015

8.7K
Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies09:29

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Published on: September 30, 2016

14.5K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.5K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
13.4K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.1K