Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endonuclear lipids in liver cells.

Sabina M Maté1, Rodolfo R Brenner, Ana Ves-Losada

  • 1Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Facultad de Ciencias Médicas, UNLP-CONICET, calles 60 y 120, Argentina.

Canadian Journal of Physiology and Pharmacology
|August 12, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insights into the Protein-Lipid Interaction of Perivitellin-2, an Unusual Snail Pore-Forming Toxin.

Toxins·2025
Same author

Understanding the effects of omega-3 fatty acid supplementation on the physical properties of brain lipid membranes.

iScience·2024
Same author

Exercise-induced cardiac mitochondrial reorganization and enhancement in spontaneously hypertensive rats.

Pflugers Archiv : European journal of physiology·2024
Same author

Histidine 19 Residue Is Essential for Cell Internalization of Antifungal Peptide SmAP<sub>α1-21</sub> Derived from the α-Core of the <i>Silybum marianum</i> Defensin DefSm2-D in <i>Fusarium graminearum</i>.

Antibiotics (Basel, Switzerland)·2022
Same author

Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains.

Methods in molecular biology (Clifton, N.J.)·2021
Same author

Nuclear-lipid-droplet proteome: carboxylesterase as a nuclear lipase involved in lipid-droplet homeostasis.

Heliyon·2021
Same journal

Next-generation therapeutics and renaissance of legacy drugs targeting the endothelin system.

Canadian journal of physiology and pharmacology·2026
Same journal

Exploring the causal relationship between inflammatory cytokines and diabetic foot ulcer: A bidirectional Mendelian randomization and clinical validation study.

Canadian journal of physiology and pharmacology·2026
Same journal

TRPV1, Endocannabinoid, and Opioid Systems in Analgesia: Molecular Mechanisms and Drug Development Strategies.

Canadian journal of physiology and pharmacology·2026
Same journal

Association of stromal cell derived factor 1α and interferon-γ induced protein 10 with Fontan pathophysiology.

Canadian journal of physiology and pharmacology·2026
Same journal

Incretins for Type 2 Diabetes.

Canadian journal of physiology and pharmacology·2026
Same journal

Glucagon-like peptide 1 receptor agonists: anti-inflammatory effects in cardiovascular diseases.

Canadian journal of physiology and pharmacology·2026
See all related articles

Lipids in rat liver nuclei, even after membrane removal, show a composition similar to the whole cell. This suggests the liver

Area of Science:

  • Cell Biology
  • Biochemistry
  • Lipidomics

Background:

  • Lipids are essential components of cellular membranes.
  • Lipids also play crucial roles within membrane-depleted nuclei.

Purpose of the Study:

  • To investigate the lipid composition of membrane-depleted rat liver nuclei.
  • To compare endonuclear lipid profiles with other cellular fractions.

Main Methods:

  • Preparation of membrane-depleted rat liver nuclei using low Triton X-100 concentrations.
  • Analysis of phospholipid and fatty acid composition.
  • Identification of phosphatidylcholine molecular species.

Main Results:

  • Phospholipids, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and sphingomyelin, were identified.

Related Experiment Videos

  • Predominantly unsaturated fatty acids, particularly n-6 series (arachidonic and linoleic acids), were found.
  • The fatty acid composition and phosphatidylcholine molecular species distribution mirrored those of the whole nucleus and other cellular components.

    • Conclusions:

    • The lipid composition of membrane-depleted rat liver nuclei is largely consistent with the overall liver cell profile.
    • Endonuclear lipid pools appear to be primarily influenced by the liver's organ-specific lipid characteristics.